HSV Acyclovir Drug Resistance by Sanger Sequencing

General Information

Lab Name

HSV Acyclovir Drug Resistance

Lab Code

HSVDR

Epic Name

(not orderable in Epic)

Description

The test is designed to detect and characterize Acyclovir drug resistance associated with specific mutations found in the Thymidine Kinase (UL23) gene of both HSV Type 1 and HSV Type 2. These mutations can lead to a reduced or absent response to Acyclovir, a commonly used antiviral treatment.

This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.

Note: This assay is no longer available as a standalone test. Refer to the following alternatives:

Synonyms

Acyclovir, Drug Resistance, HSV, Sanger

Components

Code	Name
HSVSPC	HSV Acyclovir Sample Type
HSVDRM	HSV Mutations Associated with Acyclovir Resistance
HSVNDR	Other HSV Mutations Causing Amino Acid Changes
HSVACY	HSV Acyclovir Resistance
HSVDRI	HSV Acyclovir Resistance Interpretation

Interpretation

Method

Sanger Sequencing

Reference Range

See individual components

Ordering & Collection

Specimen Type

Swabs, Blood, Spinal Fluid, BAL, Eye Fluid

Collection

Collect ONE of the following:

Swabs in 1.5 mL VTM
3 mL blood in LAVENDER, RED, SST, PPT tube
1 mL Spinal Fluid
1 mL BAL

Unacceptable: GREEN TOP, Extracted nucleic acid from an outside laboratory.

Quantity

requested: 1.5 mL plasma/serum/CSF/BAL or swabs in 1.5 mL VTM
minimum: 1 mL plasma/serum/CSF/BAL or swabs in 1 mL VTM

Processing

Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

CSF or Clear non-cellular fluid: If CSF has cellular material, centrifuge & aliquot clear CSF. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Infectious Disease Division (IDD) - Virology 1601 Lind Ave SW Renton, WA 98057	Phone: 206-685-6656 option 8 Hours of Operation: Mon–Fri: 7:00 AM–7:00 PM Sat: 9:00 AM–4:00 PM

Laboratory Test Guide