HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
General Information
Lab Name
HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
Lab Code
HSVDRR
Epic Name
HSV Acyclovir Resistance with Rapid PCR, Swab/CSF
Description
HSV Semi-Quantitative Rapid PCR, Swab/CSF, performed using the DiaSorin Simplexa™ HSV-1 & HSV-2 Direct FDA-approved assay HSV SemiQnt Rapid PCR, Swab/CSF [RPDHSV].
For positive specimens with a PCR Cycle Threshold (Ct) value <35.0, HSV Acyclovir Drug Resistance testing is reflexively performed. The HSV Acyclovir Drug Resistance assay detects and characterizes resistance-associated mutations in the Thymidine Kinase (UL23) gene of HSV-1 and HSV-2.
Note: The HSV Acyclovir Drug Resistance HSV Acyclovir Drug Resistance by Sanger Sequencing [HSVDR] assay is no longer orderable as a standalone test. To add HSVDR to a previous RPDHSV, use LADDON for SPS addon. LADDON HSVDR only to the following resulted test:
- HSV SemiQnt Rapid PCR, Swab/CSF [RPDHSV] (CSF/swabs), if CTHSV1 or CTHSV2 Ct value is <35.0
Synonyms
Acyclovir, Drug Resistance, Herpes Simplex Virus by PCR, HSV, HSV DNA, HSV Qualitative PCR, PCR for Herpes Simplex, Sanger
Components
| Code | Name |
|---|---|
| RHSVSD | HSV Rapid PCR Specimen Description |
| RHSV1 | HSV 1 Rapid PCR Result |
| CTHSV1 | HSV 1 Rapid CT Value |
| RHSV2 | HSV 2 Rapid PCR Result |
| CTHSV2 | HSV 2 Rapid CT Value |
| RHSVIN | HSV Rapid PCR Interpretation |
| HSVDRS | HSV Drug Resistance Status |
Interpretation
Method
This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.
Reference Range
See individual componentsOrdering & Collection
Specimen Type
Collection
Swab: Acceptable specimen types are cutaneous and mucocutaneous swabs stored in UTM, Remel M4, Remel M4RT, Remel M5, or Remel M6 transport media. Follow the manufacturers’ package inserts for collection media and acceptable swab types. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing.
CSF: 0.75 mL in sterile container
Unacceptable samples: Calcium alginate swabs, as they may contain substances that inhibit PCR testing.
Quantity
requested: 0.75 mL
minimum: 0.5 mL
Processing
HSVDRR specimen processing:
- Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
- CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
Outside Laboratory: Ship samples on dry ice. If -60 to -85°C freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7 days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Clinical Virology (CVIR)
Performing Location(s)
| CL |
Infectious Disease Division (IDD) - Virology
1601 Lind Ave SW |
Phone: 206-685-6656 option 8 |
|---|
Frequency
Rapid PCR is performed daily except Sunday. Sequencing is performed Wednesday.
Available STAT?
No