JAK2 V617F DNA Reflexive
General Information
Lab Name
JAK2 V617F DNA Reflexive
Lab Code
JAKRFX
Epic Name
JAK2 V617F DNA Reflexive
Description
Initial testing is performed to qualitatively evaluate for the JAK2 V617F mutation. If this is negative, Calreticulin exon 9 mutation [CALR] testing will be reflexively ordered (with additional charge). If CALR screening is also found to be negative, the MPL sequencing by NGS [HCAPSG] will be reflexively ordered (with additional charge). This assay does not evaluate for other JAK2 mutations including exon 12 nor other exons. If indicated, JAK2 single gene sequencing by NGS (HCAPSG) is suggested for evaluation of these potential variants and should be separately ordered.
Hematopathology test request form found at Request Form
**Due to staffing challenges, MPL turn-around-time is extended to 14 to 25 days; please call the lab if you have urgent testing needs”.
Synonyms
CALR, Calreticulin, Essential Thrombocythemia, ET, Idiopathic Myelofibrosis, JAK2 Val617Phe mutation, Janus tyrosine kinase 2, MPL, MPL exon10, Myelofibrosis, Myeloproliferative Neoplasm, PMF, Polycythemia, Polycythemia Vera, Primary Myelofibrosis, PV, W515K, W515L
Components
| Code | Name |
|---|---|
| JAKSD | JAK2 V617F Specimen Description |
| JAKRES | JAK2 V617F Results |
| JAKINT | JAK2 V617F Interpretation |
| JAKMTH | JAK2 V617F Method |
| JAKGRN | JAK2 V617F Case Number |
Interpretation
Method
PCR amplification of JAK2 from genomic DNA, specific for V617F point mutation. May reflexively include allele specific PCR amplification of CALR and MPL exon 10 next-generation sequencing from genomic DNA.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Preferred:
Blood: 6mL in Lavender Top (EDTA)
Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Slides: 10 unstained PB or BM aspirate smears
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.
Extracted DNA will only be accepted from CLIA certified laboratories.
Tissue from stained or cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Decalcified or unacceptable fixatives
Forms & Requisitions
Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
Quantity
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/ Slides: 5 slides
Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.
Performance
LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
| Other |
Hematopathology, Molecular
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology, Molecular hours: |
|---|
Frequency
Monday, Wednesday and Friday: Results available within 24 hours unless reflexed to MPL, then results within 12 - 14 days from start of testing. Cases requiring repeat testing, review, or addressing other considerations may have a delay of ~ 12 additional days.
Available STAT?
No