Mycobacterium chimaera DNA detection by PCR
General Information
Lab Name
Mycobacterium chimaera DNA detection by PCR
Lab Code
MCHDNA
Epic Name
Mycobacterium chimaera DNA detection by PCR
Description
Mycobcaterium chimaera DNA detection by PCR is only available upon request as a reflexive test if a 3-way split ID of “Mycobacterium chimaera or Mycobacterium intracellularae or Mycobacterium paraintracellularae” is reported from tissue/fluid or a pure culture.
Results in 2-3 business days after reflex.
For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website
Synonyms
Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB sequencing, M. avium complex, MAC, MAI PCR, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterium chimaera, Mycobacterium identification
Components
| Code | Name |
|---|---|
| MCSRRS | M chimaera PCR: Detection |
| MCHSI | M chimaera PCR: Specimen Description |
| MCHSPI | M chimaera PCR: External Identifier |
| MCHSR | M chimaera PCR: Special Requests |
| MCHSC | M chimaera PCR: Specimen Comments |
| MCHNAE | M chimaera PCR: Specimen DNA Extraction |
| MCHREV | M chimaera PCR: Pathologist Review |
| MCHME | M chimaera PCR: Method Note |
Interpretation
Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Acceptable Specimens
For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.
- Nontuberculous Mycobacteria DNA Detection [NTMDNA]
- Mycobacterium avium complex DNA Detection [MAVDNA]
- Bacterial PCR reflex NGS [BCTDNA]
Pure Culture
- Plate/slant: should have visible growth
- Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
- A mixed culture will be subject to an additional charge
Optimal Quantity
For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.
Handling Instructions
Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.
Shipping/Handling
- Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
- Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.
*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
Quantity
requested: See Optimal Quantity above
minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.
Processing
UWMC/HMC: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| UW-MT |
Microbiology, Molecular Diagnostics
206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology Performing Lab Address Clinical Microbiology Lab, NW177 |
Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers:
For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190 |
|---|
Frequency
Results in 2-3 business days after reflex.
Available STAT?
No