Mycobacterium chimaera DNA detection by PCR
General Information
Lab Name
Mycobacterium chimaera DNA detection by PCR
Lab Code
MCHDNA
Epic Name
(not orderable in Epic)
Description
Mycobacterium chimaera DNA detection by PCR is only available upon request as a reflexive test if a 3-way split ID of “Mycobacterium chimaera or Mycobacterium intracellularae or Mycobacterium paraintracellularae” is reported from tissue/fluid or a pure culture.
Results in 2-3 business days after reflex.
Synonyms
Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB sequencing, M. avium complex, MAC, MAI PCR, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterium chimaera, Mycobacterium identification
Components
| Code | Name |
|---|---|
| MCSRRS | M chimaera PCR: Detection |
| MCHSI | M chimaera PCR: Specimen Description |
| MCHSPI | M chimaera PCR: External Identifier |
| MCHSR | M chimaera PCR: Special Requests |
| MCHSC | M chimaera PCR: Specimen Comments |
| MCHNAE | M chimaera PCR: Specimen DNA Extraction |
| MCHREV | M chimaera PCR: Pathologist Review |
| MCHME | M chimaera PCR: Method Note |
Interpretation
Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Reference Range
See individual componentsGuidelines
Ordering & Collection
Specimen Type
Collection
Acceptable Specimens
For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.
- Nontuberculous Mycobacteria DNA Detection [NTMDNA]
- Mycobacterium avium complex DNA Detection [MAVDNA]
- Bacterial PCR reflex NGS [BCTDNA]
Pure Culture
- Plate/slant: should have visible growth
- Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
- A mixed culture will be subject to an additional charge
Optimal Quantity
For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.
Handling Instructions
Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.
Shipping/Handling
- Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
- Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.
*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
Quantity
requested: See Optimal Quantity above
minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.
Processing
UWMC/HMC/NW: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| CL |
Molecular Microbiology
206-598-5735 Shipping Address Attn: Molecular Microbiology Performing Lab Address UW Medicine Central Laboratory |
We receive specimens 7 days a week, 365 days a year. Please e-mail us with any questions or comments you have, so that we can provide you with the best possible service. Our e-mail is monitored in real time, and your inquiry will be answered as soon as possible. e-mail: molmicdx@uw.edu For urgent inquiries, billing inquiries, or requests for faxed reports, please contact our Client Services Department at (206) 598-5735 or (800) 713-5198. |
|---|
Frequency
Results in 2-3 business days after reflex.
Available STAT?
No