Respiratory TB PCR

General Information

Lab Name

Respiratory TB PCR

Lab Code

MTBP

Epic Name

Respiratory TB PCR

Description

This test must be ordered with an AFB culture with stain (AFBHSC). Exception: outside locations that perform AFB culture may submit sediments of specimens concentrated for AFB culture for testing. This test is reflexively ordered on the first sputum for AFB culture on HMC, UWMC-ML and UWMC-NW patients, except cystic fibrosis patients who have the test performed by request only. Additional tests can be ordered by request as long as the patient has not been on anti-TB medications for longer than 3 days. Reflexive testing is repeated every 28 days on TB negative patients. See also TBCDNA for TB PCR performed at UW-MT Microbiology. For request on FLUIDS or TISSUE see TBCDNA. Specimen deadlines for same day results are 7 AM @UW-MT and 9 AM @HMC. This test detects M. tuberculosis complex DNA. Specimens are concentrated and cultured (see AFBHSC).

Synonyms

AFB-Amplified, AMTD, Cepheid, GeneXpert, M. Tuberculosis, MTD, Mycobacteria, PCR-TB, Xpert

Components

Code Name
SDES Specimen Description
SREQ Special Requests
MTBPR MTBC PCR Result
RPT Report Status

Interpretation

Method

Nucleic Acid Amplification(Cepheid GeneXpert)

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type

Sputum, Bronchoalveolar Lavage (BAL), Brochial Washings

Collection

Deep cough or induced sputum specimens, bronchoalveolar lavage, or bronchial washings

Handling Instructions

Specimen must be refrigerated within 30 minutes and received within 5 days of collection.

Quantity

requested: 5-10 mL Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture
minimum: 2 mL (suboptimal) Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture

Processing

Specimens are stored refrigerated until concentrated.

Performance

LIS Dept Code

Microbiology (MC)

Performing Location(s)

HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency

Daily

Available STAT?

No

Billing & Coding

CPT codes

87556

LOINC

14556-5

Interfaced Order Code

UOW2952