SARS-CoV-2 and Influenza A&B Qual PCR
General Information
Lab Name
SARS-CoV-2 and Influenza A&B Qual PCR
Lab Code
NCVFLU
Epic Name
SARS-CoV-2 and Influenza A&B Qual PCR
Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® Flu A/B/RSV assay for the rapid detection and differentiation of human influenza A/B, and respiratory syncytial virus (RSV) using real-time PCR. The Hologic Emergency Use Authorization (EUA) SARS-CoV-2 Real-time RT-PCR assay is used for the detection of COVID-19 virus (SARS-CoV-2) RNA.
The Panther Fusion® Flu A/B/RSV assay targets the influenza A viruses (matrix gene), the influenza B viruses (matrix gene) and RSV (matrix gene). Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
The Hologic Emergency Use Authorization (EUA) SARS-CoV-2 Real-time RT-PCR assay targets two conserved regions of the SARS-CoV-2 (the causative agent for COVID-19) ORF1ab gene. The two regions are not differentiated; amplification of either or both regions is a presumptive positive (detectable) test result and amplification of neither target results a negative (non-detectable) test result
Components
| Code | Name |
|---|---|
| NCVFLS | SARS-CoV-2 and Flu Specimen Type |
| NCVRT | SARS CoV 2 (COVID 19) Qual PCR Result |
| NCVINI | SARS CoV 2 (COVID 19) Qual PCR Interpretation |
| FLAPCR | Influenza A PCR Result |
| CTFLA | Influenza A Ct Value |
| FLBPCR | Influenza B PCR Result |
| CTFLB | Influenza B Ct Value |
| FLUINI | Influenza A&B Qual PCR Interpretation |
Interpretation
Method
PCR
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Acceptable Specimens and Media
We prefer nasopharyngeal or oropharyngeal swab in Universal Transport Media (UTM) or viral transport medium (VTM).
Acceptable specimen types include nasopharyngeal or oropharngeal swab in UTM/VTM, nasopharyngeal swabs in M4 or M6 media, nasal swabs in UTM/VTM.
Collection
Instructions for Nasopharyngeal Swab: Gently insert mini-tipped flocked nasopharyngeal swab (swab on flexible plastic shaft) through the nostril and into the nasopharynx, reaching the posterior nasopharynx. Leave swab in place for 2-3 seconds then rotate completely around for 10-15 seconds. Remove swab and repeat the same process in the other nostril with the same swab.
After the second swab is completed, immediately place into the sterile vial containing media (UTM is preferred). Make sure that the swab is fully immersed in media, and that the shaft is short enough to completely tighten the cap. Obtaining columnar epithelial cells will enhance reliability of viral detection.
Shipping
Send to the laboratory as soon as possible. Sample may be stored at 2-8°C for up to 72 hours of collection. Ship immediately to lab at 2-8°C (ice pack). If collection to receipt in the lab will exceed 72 hours freeze at -10°C or colder and ship on dry ice. Do not freeze/thaw.
Important notes
Leaking samples will not be processed.
We currently cannot accept at-home collected swabs and await further FDA guidance on this issue.
Handling Instructions
Send specimens to
UW Medicine
Department of Laboratory Medicine
1959 NE Pacific Street, room NW220
Seattle, WA 98195
Tel: (206) 520-4600 or 1 (800) 713-5198
Additional information
Additional information on ordering, collection, and shipment can be found at https://depts.washington.edu/uwviro/order/
For all questions, contact Client Support Services (available 24/7):
Phone: (206) 520-4600 or 1 (800) 713-5198
Fax: (206) 520-4903
Email: commserv@uw.edu
Quantity
requested: 0.5mL
minimum: 0.5mL
Processing
Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
| CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
|---|
Frequency
Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?
No