Fungal DNA Detection by ITS Next Gen Seq
General Information
Lab Name
Fungal DNA Detection by ITS Next Gen Seq
Lab Code
NGSITS
Epic Name
(not orderable in Epic)
Description
Fungal ITS Next Generation Sequencing is only available as reflexive test from Fungal PCR reflex NGS [FUNDNA] when there is evidence of multiple fungal templates present.
Results in 10 business days after reflex from Fungal PCR reflex NGS [FUNDNA]
Broad range PCR and Sanger sequencing can be used to perform accurate genus- or species-level taxonomic assignment for several fungal gene targets. Unfortunately, when PCR amplification is performed on polymicrobial specimens, the presence of multiple templates causes interference during Sanger sequencing such that results are generally uninterpretable. In contrast, next-generation sequencing methods provide individual sequence data for large numbers of DNA templates, allowing each to be classified independently. This permits fungal species identification for samples from which usable data cannot be obtained by conventional clinical methods.
Synonyms
broad range fungal PCR, broad range fungi PCR, fungal identification, fungal ITS, Fungal PCR, fungal sequencing, Fungi PCR, fungi sequencing, ITS deep sequencing, ITS PCR, ITS sequencing, molecular fungal, molecular fungi, Next Generation Sequencing, NGS, polymicrobial sequencing, universal fungal PCR, universal fungi PCR
Components
| Code | Name |
|---|---|
| NGITSI | Fungal ITS Gene Next Gen Seq Specimen |
| NGITRS | Fungal ITS Gene Next Gen Seq Results |
| NGITIN | Fungal ITS Gene Next Gen Seq Interpretation |
| NGITSR | Fungal ITS Gene Next Gen Seq Requests |
| NGITME | Fungal ITS Gene Next Gen Seq Method Note |
Interpretation
Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Reference Range
See individual componentsGuidelines
Ordering & Collection
Specimen Type
Collection
Processing
This only available as a reflexive test, please refer to Fungal PCR reflex NGS [FUNDNA].
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| CL |
Molecular Microbiology
206-598-5735 Shipping Address Attn: Molecular Microbiology Performing Lab Address UW Medicine Central Laboratory |
We receive specimens 7 days a week, 365 days a year. Please e-mail us with any questions or comments you have, so that we can provide you with the best possible service. Our e-mail is monitored in real time, and your inquiry will be answered as soon as possible. e-mail: molmicdx@uw.edu For urgent inquiries, billing inquiries, or requests for faxed reports, please contact our Client Services Department at (206) 598-5735 or (800) 713-5198. |
|---|
Frequency
Available STAT?
No