Protein C Antigen
General Information
Lab Name
Protein C Ag
Lab Code
PCAG
Epic Name
Protein C Antigen
Description
Protein C Antigen test is used to diagnose congenital Protein C deficiency, particularly to distinguish type I from type II deficiency. In type I deficiency, both Protein C Antigen and Protein C Activity (functional) levels are decreased. In type II deficiency, Protein C activity levels are decreased with normal antigen levels. The preferred initial test to screen for congenital protein C deficiency is functional Protein C Activity test (see PCCLOT).
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.**
References
Stago Asserachrom Protein C package insert, revised October 2004
Interpretation
Method
Enzyme Linked Immunosorbent Assay (ELISA)
Reference Range
Units: %
| Female | Male | ||
|---|---|---|---|
| Age | Range | Age | Range |
| 0-29d | 20-150 | 0-29d | 20-150 |
| 1m-2m | 21-150 | 1m-2m | 21-150 |
| 3m-5m | 28-150 | 3m-5m | 28-150 |
| 6m-11m | 37-150 | 6m-11m | 37-150 |
| 1y-5y | 40-150 | 1y-5y | 40-150 |
| 6y-9y | 45-150 | 6y-9y | 45-150 |
| 10y- | 65-150 | 10y- | 65-150 |
Effective date: 07/01/2004
Ref. Range Notes
Elevated Protein C is not associated with thrombosis or bleeding.
Interferences and Limitations
The presence of anti-rabbit antibodies in certain subjects may lead to aberrant results. Patients on coumadin therapy may see decrease in protein C antigen because of the vitamin K antagonistic effect of the drug.
Ordering & Collection
Specimen Type
Collection
3 or 5 mL BLUE TOP (CITRATE) tube
Approval Required
Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.
Handling Instructions
The Laboratory MUST process specimen, within 4 hrs of blood collection.
Quantity
requested: entire specimen
Processing
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.**
Approval is NOT required for hospital outpatients, clinic patients or outside clients.
UW-MT Instructions: Take specimen to UW-MT Coag lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag."
HMC Instructions: Take specimen to HMC Coaglab
Note: Login PCAG 'only when a Protein C Antigen' is specified. Otherwise see Protein C Activity (PCCLOT).
Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.
Performance
LIS Dept Code
Coagulation (COAG)
Performing Location(s)
| HMC |
Coagulation
206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420 |
|---|
Frequency
Run on Wednesday and reported by end of day.
Available STAT?
No
Billing & Coding
CPT codes
85302
LOINC
Interfaced Order Code
UOW1154
Interfaced Result Code
UOW1154