BCR/ABL p190, Quant by RT PCR
General Information
Lab Name
BCR/ABL p190, Quant by RT PCR
Lab Code
QP190
Epic Name
BCR/ABL p190 Quantitative
Description
The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR.
Reportable Range: 0.01 – 100%
Limit of Detection: 0.002%
Limit of Quantification: 0.01%
Precision near LOD (CV): 24%
Stability is 4 days, specimens received after 4 days will be rejected.
Hematopathology test request form can be found at Request Form.
Synonyms
B-ALL, B-Cell Acute Lymphoblastic Leukemia, BALL, P190, QP190, Quantitative BCR/ABL p190, t(9;22)
Components
| Code | Name |
|---|---|
| P190SD | BCR/ABL p190 Specimen Desc |
| P190RS | BCR/ABL p190, Quant by RT PCR |
| P190RA | BCR/ABL p190 Ratio % |
| P190IN | BCR/ABL p190 Interpretation |
| P190M | BCR/ABL p190 Method |
| P190GR | P190 Case Number |
Interpretation
Method
LDT, RT-PCR
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Blood: 6mL in LAVENDER top (EDTA) (minimum total of 5 million WBC)
Bone Marrow: 1-2 mL in a LAVENDER top (EDTA) (minimum total of 5 million WBC)
Unacceptable:
Specimens with low volume or inadequate WBC.
Specimens received beyond stability limit.
Tissue, Decalcified or unacceptable fixatives
Please note: While green top heparin tubes are accepted for testing, there is documentation that heparin can interfere with some PCR.
Forms & Requisitions
Handling Instructions
Samples should be transported at RT and arrive in lab within 24 hours.
If sample is expected to arrive in the HP lab beyond 24 hours, store and transport at 2 – 6˚C.
Quantity
requested: Blood: 6mL in EDTA, Bone Marrow: 2mL in EDTA
minimum: Blood: 4mL in EDTA, Bone Marrow: 1mL in EDTA
Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit is 4 days, samples beyond this limit will be rejected.
Performance
LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
| Other |
Hematopathology, Molecular
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology, Molecular hours: |
|---|
Frequency
Monday, Wednesday, Friday: Results available within 96hrs from receipt.
Available STAT?
No