Encephalitis Meningitis PCR, CSF (Sendout)
General Information
Lab Name
Encephalitis Meningitis PCR, CSF (Sendout)
Lab Code
RCEMPC
Epic Name
Encephalitis Meningitis PCR, CSF (Sendout)
External Test Id
LAB2421
Description
Description: May detect the following agents:
- Cytomegalovirus
- Enterovirus
- Herpes Simplex Virus 1 and 2
- Human Herpes Virus 6
- Human parechovirus
- Varicella zoster virus
- Cryptococcus gattii*/neoformans
- Escherichia coli K1
- Haemophilus influenzae*
- Listeria monoctyogenes*
- Neisseria meningitidis
- Streptococcus agalactiae (group B Streptococcus)
- Streptococcus pneumoniae
*WA State DOH Notifiable Conditions
Ordering Note: Approval by the Laboratory Medicine Resident (LMR) on-call is required for all orders. Molecular testing does not replace bacterial or fungal cultures. The Biofire Meningitis/Encephalitis has demonstrated false positive and false negative results. See the following preferred tests for specific agents: HSV, VZV, Cryptococcus, bacteria. Other specific tests are available for: CMV, HHV6, Enterovirus/Parechovirus, fungal culture, bacterial PCR, Cryptococcus PCR, pneumococcal urine antigen.
Synonyms
Cryptococcus gattii/neoformans, CSF meningitis/encephalitis PCR Qual Panel, Cytomegalovirus, Encephalitis, Enterovirus, Escherichia coli K1, group B Streptococcus, Haemophilus influenzae, Herpes Simplex Virus 1 and 2, Human Herpes Virus 6, Human parechovirus, LAB2421, Listeria monoctyogenes, Meningitis, Neisseria meningitidis, pneumococcus, Streptococcus agalactiae, Streptococcus pneumoniae, Varicella zoster virus
Components
Interpretation
Method
Molecular method by BioFire FilmArray (Film Array)
Reference Range
See individual components
Ref. Range Notes
Reference Range: Negative
Positive or negative is reported for each infectious agent.
Guidelines
Ordering & Collection
Specimen Type
Collection
1 mL CSF collected in a sterile container
- Note: Clotted CSF is not acceptable for this test and will be rejected.
- Testing will only be performed on CSF from lumbar puncture. CSF shunt fluid is not an appropriate sample for this test.
Approval Required
Laboratory Medicine Resident (LMR) approval is required.
Handling Instructions
Outside Laboratories: Freeze CSF in a sterile container at -20°C.
Stability: Frozen: 4 months; Refrigerated: 7 days; Ambient: 24 hours.
Reject Due To: Clotted CSF is not acceptable for this test and will be rejected.
Quantity
requested: 1 mL CSF
minimum: 0.5 mL CSF
Processing
Freeze CSF in a sterile container at -20°C. Notify the appropriate bench of an incoming sample or add-on order. This test should be prioritized for immediate sendout.
- UWMC: Freeze sample in Sendouts rack and notify UW-MT Sendouts.
- HMC: Freeze sample in the Micro rack and notify HMC Microbiology.
Sendouts (UWMT)/Microbiology (HMC):
- Order SCHL Test: LAB2421.
- Interfaced: Yes. [Interface: 601; Department: SOSC]
- Send samples as received to Seattle Children's Hospital Lab via Delivery Express courier with a copy of the MayoAccess Interface Packing List.
Stability: Frozen: 4 months; Refrigerated: 7 days; Ambient: 24 hours.
Reject Due To: Clotted CSF is not acceptable for this test and will be rejected.
Performance
LIS Dept Code
SCHL Interfaced Sendouts (SOSC)
Performing Location(s)
| Sendout |
Seattle Children's Hospital Department of Laboratories
206-987-2617 4800 Sand Point Way NE |
|---|
Frequency
Performed: 24/7. Turn around time: 2 hours.
Available STAT?
No