Trypanosoma cruzi Antibody Panel (Sendout)

General Information

Lab Name

Trypanosoma cruzi Antibody Panel (Sendout)

Lab Code

RCRUZI

Epic Name

Trypanosoma cruzi Antibody Panel (Sendout)

External Test Id

CRUZI

Description

Useful For: Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease) using two different methods.

    Ordering Guidance: This panel includes two independent T. cruzi antibody assays performed using different methods, as recommended by the CDC for serologic diagnosis of Chagas disease¹. If results are discordant, additional testing may be required for resolution. Contact the Laboratory Medicine Resident or Microbiology fellow on-call for guidance on subsequent testing.

    References

    1. Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
    2. Mayo Clinic Labs Clinical & Interpretive Information: Trypanosoma cruzi (Chagas) Antibody Panel, Serum

    Synonyms

    American trypanosomiasis, Chagas, Chagas disease, CRUZI, Kissing bug, RCHAGS, Reduviid, T. cruzi, Triatoma, Trypanosoma, Trypanosomiasis

    Components

    Code Name
    RCRUZT Trypanosoma cruzi Total Antibody, EIA
    RCRUZG Trypanosoma cruzi IgG, LFA
    RCRZIN Trypanosoma cruzi Interpretation

    Interpretation

    Method

    Enzyme-Linked Immunosorbent Assay (ELISA), Lateral Flow Assay (LFA)

    Reference Range

    See individual components

    Ref. Range Notes

    Reference Values:

    Trypanosoma cruzi Total Antibody, EIA: Negative
    Trypanosoma cruzi IgG, LFA: Negative

    Additional interpretive information is available on the Mayo website.²

    Interferences and Limitations

    Cautions:

    False-positive results may occur in patients infected with Leishmania species or other Trypanosoma species, including Trypanosoma rangeli. Additionally, false-positive results with the T cruzi lateral flow assay have been detected in patients with hepatitis C, toxoplasmosis, or syphilis.

    A diagnosis of chronic Chagas disease requires both clinical evaluation (including exposure history) and laboratory results. Chagas disease should not be diagnosed based on a single serologic result alone.

    A single negative result does not exclude the diagnosis of Chagas disease as antibodies to the pathogen may not yet be detectable. Sensitivity of the assay may be decreased in significantly immunosuppressed patients.

    Guidelines

    Ordering & Collection

    Specimen Type

    Blood

    Collection

    3 mL blood in RED or GOLD SST tube

    Handling Instructions

    Outside Laboratories: Centrifuge sample and aliquot serum to a separate plastic vial. Freeze serum at -20°C while awaiting shipment. Transport on dry ice.

    Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.

    Reject Due To: Gross hemolysis, gross lipemia.

    Quantity

    requested: 0.7 mL serum
    minimum: 0.5 mL serum

    Processing

    Centrifuge sample and aliquot serum to a separate plastic vial. Freeze serum at -20°C.

    Sendouts:

    • Order Mayo Test: CRUZI
    • Interfaced: Yes [Interface: 601; Worksheet: MAFZ]

    Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.

    Reject Due To: Gross hemolysis, gross lipemia.

    Performance

    LIS Dept Code

    Sendouts Mayo Lab (FZ) (MAFZ)

    Performing Location(s)

    Sendout Mayo Clinic Laboratories (Superior Drive)
    800-533-1710

    3050 Superior Drive NW
    Rochester, MN 55901

    Frequency

    Performed: Monday. Reported: 1-8 days from sample receipt at Mayo Clinic Labs.

    Available STAT?

    No

    Billing & Coding

    CPT codes

    86753x2

    LOINC

    23785-9

    Interfaced Order Code

    UOW6010

    Interfaced Result Code

    UOW6013