Hepatitis E Virus Antibodies (Sendout)

General Information

Lab Name

Hepatitis E Virus Antibodies

Lab Code

RHEPEA

Epic Name

Hepatitis E Virus Antibodies (Sendout)

External Test Id

HEVG, HEVM

Description

Useful For:

  • Hepatitis E, IgG: Diagnosis of past exposure to Hepatitis E virus.
  • Hepatitis E, IgM: Diagnosis of acute or recent (<6 mo months) Hepatitis E infection.

Test Algorithm: If Hepatitis E Virus (HEV) IgM Antibody Screen is reactive or borderline, Hepatitis E IgM Confirmation (Sendout) [RHEVMC] will be performed.

References

  1. Mayo Clinic Laboratories Hepatitis E Virus IgG Clinical & Interpretive Information
  2. Mayo Clinic Laboratories Hepatitis E Virus IgM with reflex Clinical & Interpretive Information

Synonyms

Anti-HEV, Hepatitis E IgG & IgM, Hepatitis E Screen, HEV, HEVG, HEVM

Components

Code Name
RHEIGG Hepatitis E Virus, IgG
RHEIGM Hepatitis E Virus, IgM

Interpretation

Method

Enzyme Immunoassay (EIA)-Screening Procedure

Confirmatory IgM, if performed: Line Immunoassay (LIA)

Reference Range

See individual components

Interferences and Limitations

Cautions:

IgG: A negative test result does not exclude the presence of recent hepatitis E infection (<2 month duration), especially in immunocompromised patients. Repeat testing for anti-hepatitis E virus (HEV) IgM and anti-HEV IgG in 1 to 2 months is necessary for diagnosis of recent hepatitis E.

IgM: Despite having a high specificity rate, the positive predictive value of the hepatitis E virus (HEV) IgM antibody screening test may be low (ie, relatively high frequency of false-positive test results) due to low prevalence of acute hepatitis E in the patient population being screened. HEV IgM antibody confirmatory test is helpful and necessary to determine the true infection status of patients with reactive HEV IgM antibody screening test results.

A negative test result does not exclude the presence of recent hepatitis E infection, especially in immunocompromised patients. Repeat testing of serum for anti-HEV IgM in 2 to 4 weeks may be necessary for diagnosis of acute in such patients.

IgG, IgM: Performance characteristics of this assay have not been established for serum specimens that are heat inactivated, icteric, lipemic, hemolyzed, or contain particulate matter.

Guidelines

Ordering & Collection

Specimen Type

Blood

Collection

Collect 3 mL blood in RED TOP tube or GOLD TOP SST

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C while awaiting shipment. Transport samples on dry ice.

Stability: Frozen (preferred): Indefinitely; Refrigerated: 24 hours; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Quantity

requested: 1 mL serum
minimum: 0.2 mL serum

Processing

Centrifuge sample and transfer serum to a separate aliquot. Freeze sample at -20° C.

Sendouts:

  • Order Mayo Tests: HEVG and HEVM.
  • Interfaced: Yes.

Stability: Frozen (preferred): Indefinitely; Refrigerated: 24 hours; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Performance

LIS Dept Code

Performing Location(s)

Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency

Performed: Tuesday, Thursday. Report Available: 1-7 days. IgM Confirmation: 1-21 days.

Available STAT?

No

Billing & Coding

CPT codes

86790x2

LOINC

13294-4

Interfaced Order Code

UOW3969