Lymphocyte Mitogen/Antigen Stimulation (Sendout)
General Information
Lab Name
Lymphocyte Mitogen/Ag Stimulation Bty
Lab Code
RMIAGG
Epic Name
LYMPH MITOG STIM ANTIG STIM
External Test Id
LAB3317, LAB2806
Description
Description:
- The Mitogen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to non-specific stimuli (PHA and/or anti-CD3).
- The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).
Ordering Note: Non-orderable battery included in Lymphocyte Mitogen/Ag Stimulation (Sendout) [RMIAGP] package.
Synonyms
anti-CD3, Antigen stimulation, Candida Antigen Stimulation, LAB2806, LAB3317, Lymphocyte Function Analysis, Lymphocyte Mitogen Stimulation, Lymphocyte Transformation, MSS, PHA, Phytohemagglutinin, T Cell proliferation to Antigens, T Cell proliferation to Mitogens, Tetanus Stimulation
Components
| Code | Name |
|---|---|
| RMIAGR | Lymphocyte Mitogen/Ag Requested |
| RMSPHA | Mitogen Stimulation PHA |
| RMSCD3 | Mitogen Stimulation CD3 |
| RASTET | Ag Stimulation Tetanus Toxoid |
| RASCAN | Ag Stimulation Candida Ag |
| RASMSI | Mitogen/Ag Stimulation Impression |
Interpretation
Method
Lymphocyte proliferation with 3H thymidine incorporation
Reference Range
See individual components
Ordering & Collection
Specimen Type
Collection
20 mL blood in (2) 10 mL GREEN TOP (Sodium Heparin/Na Hep) tubes
- Expedite transport to the lab. Samples must arrive at SCHL within 24 hours of collection.
- AM Collections: Collect Monday - Friday.Samples collected on a Friday must arrive in SPS by 09:00 A.M. so that samples arrive at SCHL with sufficient time to make the run at noon.
- PM Collections: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
- Do not collect on or before holidays.
Unacceptable: Lithium heparin tubes (with or without gel)
Forms & Requisitions
Handling Instructions
Outside Laboratories: Due to the limited sample stability for this test, outside laboratories should arrange for testing directly with Seattle Children's Hospital Laboratory.
Quantity
requested: 20 mL whole blood (NaHep)
minimum: 20 mL whole blood (NaHep)
Processing
SPS: Expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store in ambient Sendouts rack (USENDA) while awaiting shipment.
Login: at result prompt for RMIAGR, enter Mitogen(s) and/or Antigen(s) requested by provider. (Choices: PHA, CD3, Candida, Tetanus).
Sendouts:
- Review EPIC order to confirm which mitogens and/or antigens are being requested.
- Expedite processing. Send samples as received via Delivery Express courier.
- AM Collections: Must be sent out same day and arrive in SCH Lab by noon for same-day setup.
- PM Collections: May be held at room temperature for sendout the following morning. Must arrive at SCH Lab by noon for setup.
- If samples are sent close to the noon cutoff for setup, notify SCHL Main Lab of incoming samples by calling 206-987-2617. Failure to do so may result in test cancellations.
Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
Performance
LIS Dept Code
Sendouts (SO)
Performing Location(s)
| Sendout |
Seattle Children's Hospital Department of Laboratories
206-987-2617 4800 Sand Point Way NE |
|---|
Frequency
Performed: Monday-Thursday; Friday if specimen arrives at SCHL by noon. Results available in 7-10 days.
Available STAT?
No