Lymphocyte Mitogen/Antigen Stimulation (Sendout)
General Information
Lab Name
Lymphocyte Mitogen/Antigen Stimulation (Sendout)
Lab Code
RMIAGP
Epic Name
Lymphocyte Mitogen/Antigen Stimulation (Sendout)
External Test Id
LAB3317, LAB2806
Description
Description:
- The Mitogen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to non-specific stimuli (PHA and/or anti-CD3).
- The Antigen Stimulation Study is used in the evaluation of immunodeficiency to determine the functional capabilities of peripheral blood mononuclear cells to respond to specific stimuli (Tetanus and/or Candida).
Ordering Requirements: Provider must specify which mitogens (PHA, CD3) and/or antigens (Tetanus, Candida) are required. Failure to do so may result in significant delays or cancellations.
Collection Note: Limited specimen stability. Time blood collections accordingly to ensure that samples reach the performing lab within the 24-hour stability limit.
Synonyms
anti-CD3, Antigen stimulation, Candida Antigen Stimulation, LAB2806, LAB3317, Lymphocyte Function Analysis, Lymphocyte Transformation, MSS, PHA, Phytohemagglutinin, T Cell proliferation to Antigens, T Cell proliferation to Mitogens, Tetanus Stimulation
Components
| Code | Name |
|---|---|
| RMIAGG | Lymphocyte Mitogen/Ag Stimulation Bty |
| RMIAGR | Lymphocyte Mitogen/Antigen Requested |
| RMSPHA | Mitogen Stimulation PHA |
| RMSCD3 | Mitogen Stimulation CD3 |
| RASTET | Antigen Stimulation Tetanus Toxoid |
| RASCAN | Antigen Stimulation Candida Antigen |
| RASMSI | Mitogen/Antigen Stimulation Impression |
Interpretation
Method
Lymphocyte proliferation with 3H thymidine incorporation
Reference Range
See individual components
Ref. Range Notes
Reference Values: See report.
Ordering & Collection
Specimen Type
Collection
Collection: 20 mL blood in (2) 10 mL GREEN TOP (Sodium Heparin/Na Hep) tubes
- Unacceptable: Lithium heparin tubes (with or without gel)
Collection Timing:
- AM Collections: Collect Monday - Friday. Samples collected on a Friday must arrive in SPS by 09:00 A.M. to ensure sufficient time to make the noon run at Seattle Children's.
- PM collections: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
- Do not collect on or before holidays.
Expedite transport to the lab. Samples must arrive at SCH Lab within 24 hours of collection.
Forms & Requisitions
- SCH Cell Marker Lab Requisition (to be completed by lab staff)
Handling Instructions
Vancouver Clinic (TVC) Only:
- Expedite transport to the UW Central Lab to ensure that the samples arrive at Seattle Children's lab within the 24-hour stability.
- Include the mitogen(s) and/or antigen(s) requested for each order (PHA, CD3, Tetanus, Candida). Failure to provide this information may result in delays or cancellations.
Other Outside Laboratories: Due to the limited sample stability for this test, clients should arrange for testing directly with Seattle Children's Hospital Laboratory.
Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
Quantity
requested: 20 mL whole blood (NaHep)
minimum: 20 mL whole blood (NaHep)
Processing
SPS: Short stability; expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store samples in the ambient Sendouts rack at each site (USENDA/HSENDA/CSENDA).
Result-at-Entry:
- RMIAGR (Lymphocyte Mitogen/Antigen Requested): <If not filed from Epic, enter the specific mitogens and/or antigens requested from the table below.>
Mitogens/Antigens Available:
| Mitogen | PHA |
| Mitogen | CD3 |
| Antigen | Tetanus |
| Antigen | Candida |
Sendouts (UW-MT or HMC)/Central Lab (RCLSPS):
- SEACHL Tests:
- LAB3317 (Mitogen Stimulation Study)
- LAB2806 (Antigen Stimulation Study)
- Review the Epic order or TVC manifest to confirm which mitogens and/or antigens are being requested. Complete the SEACHL Cell Marker Requisition accordingly.
- Expedite transport. Send samples as received via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
- AM Collections: Must be sent out same day and arrive in SCH Lab by noon for same-day setup.
- PM Collections: May be held at room temperature for sendout the following morning. Must arrive at SCH Lab by noon for setup.
- Samples drawn on Friday must be received at SCH lab by noon.
- Notify SCH Cell Marker Lab at 206-987-2560 if sending a sample nearing the stability limit or Friday noon cutoff.
Stability: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
Performance
LIS Dept Code
Performing Location(s)
| Sendout |
Seattle Children's Hospital Laboratories
206-987-2617 4800 Sand Point Way NE |
Room Number: FB.2.441 |
|---|
Other Locations/Notes
Seattle Children's Hospital Cell Marker Laboratory:
- Phone: 206-987-2560
Frequency
Performed: Monday-Thursday; Friday if specimen arrives at SCHL by noon. Results available in 7-10 days.
Available STAT?
No