Neutrophil Oxidative Burst (Sendout)

General Information

Lab Name

Neutrophil Oxidative Burst

Lab Code

RNOBG

Epic Name

(not orderable in Epic)

External Test Id

LAB3348

Description

Description: This test is a flow cytometric assay of neutrophil function utilizing the dye dihydrorhodamine (DHR). The assay is used to aid in the diagnosis of Chronic Granulomatous Disease (CGD); x-linked carrier states can also be distinguished.

    Ordering Note: Non-orderable battery included in Neutrophil Oxidative Burst (Sendout) [RNOBGP] package.

    Synonyms

    Chronic Granulomatous Disease (CGD), DHR, Dihydrorhodamine (DHR), LAB3348, Metabolic Burst Assay, Nitroblue Tetrazolium Dye Test, Oxidative Burst-Neutrophil, Respiratory Burst Assay

    Components

    Code Name
    RNOB Neutrophil Oxidative Burst
    RNOBIM Neut. Oxid. Burst Impression

    Interpretation

    Method

    Flow Cytometry

    Reference Range

    See individual components

    Ordering & Collection

    Specimen Type

    Blood

    Collection

    Collection: 1 mL in GREEN TOP (Sodium Heparin/Na Hep) tube

    • Unacceptable: Lithium heparin tubes (with or without gel)

    Collection Timing:

    • A.M. Samples: Collect Monday - Friday. Samples collected on a Friday must arrive at the lab by 09:00 A.M.
    • P.M. Samples: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
    • Do not collect on or before holidays.

      Expedite transport to the laboratory. Samples must arrive at the performing lab within 20 hours of collection.

      Forms & Requisitions

      Handling Instructions

      Vancouver Clinic (TVC) Only:

      • Expedite transport to the UW Central Lab to ensure that the samples arrive at Seattle Children's lab within the 20-hour* stability.

      Other Outside Laboratories: Due to the limited sample stability for this test, clients should arrange for testing directly with Seattle Children's Hospital Laboratory.

        Stability: Ambient (Required): 20 hours*; Refrigerated: Unacceptable; Frozen: Unacceptable.

        • *20 hours recommended, may be stable to 50 hours. Contact Cell Markers Lab at 206-987-2560 to confirm acceptability of samples >20 hours old.

        Quantity

        requested: 1 mL NaHep whole blood
        minimum: 0.5 mL NaHep whole blood (microtainers not accepted)

        Processing

        SPS: Short stability; expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store samples in the ambient Sendouts rack at each site (USENDA/HSENDA/CSENDA).

          Sendouts (UW-MT or HMC)/Central Lab (RCLSPS):

          • Order SEACHL Test: LAB3348
          • Expedite transport. Send samples as received via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method. Samples must be received by SCH Lab within 20 hours of collection.*
          • Samples drawn on Friday must be received at SCH lab by noon.
          • Notify SCH Cell Marker Lab at 206-987-2560 if sending a sample nearing the stability limit or Friday noon cutoff.

          Stability: Ambient (Required): 20 hours*; Refrigerated: Unacceptable; Frozen: Unacceptable.

          • *20 hours recommended, may be stable to 50 hours. Contact Cell Markers Lab at 206-987-2560 to confirm acceptibility of samples >20 hours old.

          Performance

          LIS Dept Code

          Sendouts (SO)

          Performing Location(s)

          Sendout Seattle Children's Hospital Laboratories
          206-987-2617

          4800 Sand Point Way NE
          Seattle, WA 98105

          Room Number: FB.2.441

          Frequency

          Performed: Monday - Friday at noon. TAT: 24 hours from sample receipt at Seattle Children's Hospital Laboratory.

          Available STAT?

          No

          Billing & Coding

          CPT codes

          86352

          LOINC

          30903-9

          Interfaced Order Code

          UOW3986