Neutrophil Oxidative Burst (Sendout)
General Information
Lab Name
Neutrophil Oxidative Burst (Sendout)
Lab Code
RNOBGP
Epic Name
Neutrophil Oxidative Burst (Sendout)
External Test Id
LAB3348
Description
Description: This test is a flow cytometric assay of neutrophil function utilizing the dye dihydrorhodamine (DHR). The assay is used to aid in the diagnosis of Chronic Granulomatous Disease (CGD); x-linked carrier states can also be distinguished.
Collection Note: Limited specimen stability. Time blood collections accordingly to ensure that samples reach the performing lab within the 20-hour stability limit.
Synonyms
Chronic Granulomatous Disease (CGD), DHR, Dihydrorhodamine (DHR), LAB3348, Metabolic Burst Assay, Nitroblue Tetrazolium Dye Test, Oxidative Burst-Neutrophil, Respiratory Burst Assay
Components
| Code | Name |
|---|---|
| RNOBG | Neutrophil Oxidative Burst |
| RNOB | Neutrophil Oxidative Burst |
| RNOBIM | Neut. Oxid. Burst Impression |
Interpretation
Method
Flow Cytometry
Reference Range
See individual componentsRef. Range Notes
| Normal: | Stimulation Index >20 |
Ordering & Collection
Specimen Type
Collection
Collection: 1 mL in GREEN TOP (Sodium Heparin/Na Hep) tube
- Unacceptable: Lithium heparin tubes (with or without gel)
Collection Timing:
- A.M. Samples: Collect Monday - Friday. Samples collected on a Friday must arrive at the lab by 09:00 A.M.
- P.M. Samples: Collect Monday - Thursday only. Orders collected late morning or afternoon on Fridays will be cancelled.
- Do not collect on or before holidays.
Expedite transport to the laboratory. Samples must arrive at the performing lab within 20 hours of collection.
Forms & Requisitions
- Seattle Children's Hospital Cell Marker Lab Requisition
Handling Instructions
Vancouver Clinic (TVC) Only:
- Expedite transport to the UW Central Lab to ensure that the samples arrive at Seattle Children's lab within the 20-hour* stability.
Other Outside Laboratories: Due to the limited sample stability for this test, clients should arrange for testing directly with Seattle Children's Hospital Laboratory.
Stability: Ambient (Required): 20 hours*; Refrigerated: Unacceptable; Frozen: Unacceptable.
- *20 hours recommended, may be stable to 50 hours. Contact Cell Markers Lab at 206-987-2560 to confirm acceptability of samples >20 hours old.
Quantity
requested: 1 mL NaHep whole blood
minimum: 0.5 mL NaHep whole blood (microtainers not accepted)
Processing
SPS: Short stability; expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store samples in the ambient Sendouts rack at each site (USENDA/HSENDA/CSENDA).
Sendouts (UW-MT or HMC)/Central Lab (RCLSPS):
- Order SEACHL Test: LAB3348
- Expedite transport. Send samples as received via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method. Samples must be received by SCH Lab within 20 hours of collection.*
- Samples drawn on Friday must be received at SCH lab by noon.
- Notify SCH Cell Marker Lab at 206-987-2560 if sending a sample nearing the stability limit or Friday noon cutoff.
Stability: Ambient (Required): 20 hours*; Refrigerated: Unacceptable; Frozen: Unacceptable.
- *20 hours recommended, may be stable to 50 hours. Contact Cell Markers Lab at 206-987-2560 to confirm acceptability of samples >20 hours old.
Performance
LIS Dept Code
Performing Location(s)
| Sendout |
Seattle Children's Hospital Laboratories
206-987-2617 4800 Sand Point Way NE |
Room Number: FB.2.441 |
|---|
Frequency
Performed: Monday - Friday at noon. TAT: 24 hours from sample receipt at Seattle Children's Hospital Laboratory.
Available STAT?
No