T Cell Clonality by PCR
General Information
Lab Name
T Cell Clonality by PCR
Lab Code
TCRG
Epic Name
T Cell Clonality Analysis by PCR
Description
Monoclonal populations of T lymphocytes can be identified by testing for T cell receptor gamma chain (TCRG) gene rearrangements using a multiplex polymerase chain reaction assay followed by fragment analysis by capillary electrophoresis. A polyclonal T cell population will produce a Gaussian distribution of PCR products on capillary electrophoresis, while a clonal process will produce one or two discrete peaks.
Sensitivity: This assay detects approximately 95% of T cell clones, provided that the clonal population represents at least 2-5% of the T cells in a specimen.
Hematopathology test request form found at Request Form
Synonyms
Gamma chain, T Cell Receptor
Components
| Code | Name |
|---|---|
| TCELSD | T Cell Clonality PCR Specimen |
| TCELRS | T Cell Clonality PCR Result |
| TCELIN | T Cell Clonality PCR Interp |
| TCREV | T Cell Clonality Results Reviewed by: |
| TCELM | T Cell Clonality PCR Method |
| TCRGRN | T Cell Clonality Case Number |
Interpretation
Method
Polymerase Chain Reaction (PCR)/Capillary Electrophoresis
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Preferred:
Blood: 6mL in Lavender Top (EDTA)
Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Formalin-fixed paraffin embedded tissue (FFPE/PET) block
Slides: 10 unstained
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.
Extracted DNA from CLIA certified laboratory.
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives
Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
Quantity
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides
Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.
Blood and BMA must arrive in lab by 7am and tissue by 12pm Friday to be included on Tuesday run.
Performance
LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
| Other |
Hematopathology, Molecular
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology, Molecular hours: |
|---|
Frequency
Tuesday and Thursday: Results available up to 5 days.
Available STAT?
No