Laboratory Test Guide

Place Holder for Wound Bacterial Culture and Sensitivity with Gram Stain

General Information

Lab Name

Wound Culture w/Gram Order

Lab Code

WNDPHC

Epic Name

Culture Wound, Bact w/ Stain

Description

Lab will order appropriate test based on specimen description. Specify exact source of specimen, preferably using pre-defined specimen descriptors in CPOE. Indicate in special requests if need for anaerobic cultures differs from below.

Wound Bacterial Culture and Sensitivity with Gram Stain [WNDC]: Aerobic workup only. Includes skin and superficial sites such as boil, furuncle, cyst, ulcer, superficial abrasion, first degree burn, impetigo, folliculitis, and cellulitis.

Wound Bacterial Culture and Sensitivity (Special Anaerobic) with Gram Stain [WNDANC]: Aerobic and anaerobic workup. Includes abscess, hardware, deep and/or sterile sites.

Genital Bacterial Culture and Sensitivity (Routine) without Gram Stain [GUC]: Aerobic workup of genital sites. Includes vagina, cervix, endocervix, vulva, penis, and urethra. (No gram stain).

Genital Bacterial Culture and Sensitivity (Anaerobic) with Gram Stain [GUWANC]: Aerobic and anaerobic workup of genital sites. Includes abscess, placenta, amnion/chorion, endometrium and semen.

R/O Gonorrhoeae without Gram [GCC]: R/O Neisseria gonorrheae only.

R/O Staph aureus Bacterial Culture only without Gram [STAPHC]: R/O Staph aureus only, culture and sensitivity.

For body fluids/drains see Place Holder for Body Fluid Bacterial Culture and Sensitivity with Gram Stain (includes anaerobes) [FLDPHC].

For tissues/biopsies see Tissue Bacterial Culture and Sensitivity with Gram [TISC].

For fungal cultures see Wound Fungal Culture with KOH Exam [WNDF].

For R/O yeast only see R/O Yeast Culture with Direct Exam [YSTF].

Synonyms

Abscess culture, deep wound, genital wound, skin wound, superficial wound

Components

Code	Name
SDES	Specimen Description
SREQ	Special Requests
MCCOM	MICROBIOLOGY COMMENT
RPT	Report Status

Interpretation

Reference Range

See individual components

Ordering & Collection

Specimen Type

Various

Collection

Remove surface exudate. Collect abscess fluid in syringe with Luer tip cap (remove needle) or swab in liquid Amies transport medium (ESwab) or anaerobic transport vial. Swab in Amies gel transport medium is also acceptable.

Handling Instructions

Transport Swabs ideally within 4 hours and syringe or sterile container within 2 hours at room temperature. If specimen is received >12 hours after collection, viability of organisms may be significantly reduced.

Specimens received >24 hours after collection, or collected with expired swabs/transport media will be rejected.

Specimens collected with Universal Transport Medium (UTM) are unacceptable for culture.

Quantity

requested: As much as possible. Sensitivity of culture may be improved by larger specimen volume/size. For maximal sensitivity, as applicable to the specimen, body fluids should be submitted as fluid culture (see FLDPHC) and not as a swab of the fluid.
minimum: Amies or Anaerobic swab

Processing

Performance

LIS Dept Code

Microbiology (MC)

Performing Location(s)

HMC	Microbiology 206-744-5858 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Microbiology 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195