Laboratory Test Guide

Zika, Chikungunya, and Dengue Antibody Panel (Sendout)

General Information

Lab Name

Lab Code

655

External Test Id

0093096, 2011812, MZIKV

Description

Test Information: This panel includes testing for antibodies to Zika Virus, Chikungunya Virus (ChikV), and Dengue Fever Virus. This assay should be used in patients presenting at least 2 weeks postsymptom onset or last possible exposure to Zika virus. Reverse transcription-polymerase chain reaction (RT-PCR) for Zika virus RNA detection should be ordered in patients with less than 2 weeks of symptoms or postexposure.

Tests Included:

Zika IgM Capture ELISA is used under the Food and Drug Administration’s Emergency Use Authorization.

Resources for Health Care Providers

Synonyms

0093096, 2011812, Chikungunya, CHIKV, Dengue Fever, Flavivirus, MZIKV, Zika Virus

Interpretation

Method

Chikungunya Antibodies, IgG and IgM: Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Dengue Fever Virus Antibodies, IgG and IgM: Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Zika IgM: IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA)

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type

Blood

Collection

15 mL blood in GOLD SST or RED TOP tube

Quantity

requested: Chikungunya: 1 ml serum; Dengue: 1 ml serum; Zika: 2.5 ml serum
minimum: Chikungunya: 0.15 ml serum; Dengue: 0.1 ml serum; Zika: 2.0 ml serum

Processing

Panel includes the three tests indicated below. Refer to individual test guides for additional processing information.

Chikungunya Virus IgG and IgM Antibodies (Sendout) [RCHIKV] - Refrigerated serum
Dengue Fever IgG and IgM Antibody (Sendout) [RDFAGM] - Refrigerated serum
Zika Virus Antibody, IgM (Sendout) [RZIKM] - Frozen serum

Sendouts:

Order ARUP Tests: 2011812 (Chikungunya) and 0093096 (Dengue).
Order Mayo Test: MZIKV (Zika). Provide answers to the questions (3) indicated in the general information section when ordering in MayoAccess.

Stability:

Chikungunya (ARUP 2011812): Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles); Ambient: 48 hours.
Dengue (ARUP 0093096): Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles); Ambient: 48 hours.
Zika (Mayo MZIKV): Frozen: 30 days; Refrigerated: Unacceptable; Ambient: Unacceptable.

Performance

LIS Dept Code

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108
Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901