Bartonella DNA detection by PCR
General Information
Lab Name
Bartonella DNA detection by PCR
Lab Code
BRTDNA
Epic Name
Bartonella DNA detection by PCR
Description
Detection and differentiation of Bartonella henselae and Bartonella quintana DNA.
Species of the genus Bartonella are currently recognized in growing numbers and are involved in an increasing variety of human diseases, mainly trench fever, Carrion's disease, bacillary angiomatosis, endocarditis, cat scratch dease, neuroretinities, and asymptomatic bacteremia.
Amplification-based techniques enable detection and identification of the bacteria to be performed directly from clinical specimens.
Partial ITS amplification and sequencing offers a sensitive means of intraspecies differentiation. Each Bartonella possesses a single, species-specific ITS.
ribC is highly conserved at the species level. Primer oligonucleotides designed on the basis of localized differences with the ribC DNA region are used in species-specific assays for the differentiation of B. henselae and B. quintana, the two most commonly isolated Bartonella species. The PCR assay increases the diagnostic potential to detect and differentiate Bartonella species.
For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website
Synonyms
16S PCR, 16S sequencing, Bartonella 16S, Bartonella henselae, Bartonella identification, Bartonella PCR, Bartonella quintana, Bartonella ribC, Bartonella sequencing, Cat Scratch, molecular Bartonella, ribC PCR, ribC sequencing, Trench Fever, universal PCR
Components
Interpretation
Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Acceptable specimens are listed below. Please see this page for complete specimen collection and handling instructions.
Specimens should be collected into a DNA free container labeled with at least two identifiers.
Acceptable Specimens
- Fresh frozen tissue
- Fresh frozen fluid: any body fluid is acceptable if it is not listed under Unacceptable Specimens. See common examples below.
- Formalin Fixed Paraffin-embedded tissues (FFPE, PET): blocks, scrolls, and unstained slides are acceptable
- eSwabs and UTM (universal transport media)
Common acceptable body fluid examples: cerebrospinal fluid, pleural fluid, pericardial fluid, urine, bronchial lavage, joint fluid, bone marrow, vitreous fluid, etc.
Additional Acceptable Specimens
- Sputum
Unacceptable Specimens
- Blood, serum, plasma, stool
- No citrated or heparinized solutions
- No SPS (Sodium Polyanethole Sulfonate) e.g. Wampole Isolator Tubes
- Tissues floating in formalin
- Swab/fluid collected in tube containing agar
Handling Instructions
Fresh frozen tissue/fluid specimens should be submitted and maintained on dry ice.
Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
Optimal Quantity:
- Fresh Tissue: 0.3-1.0 cm^3
- Fluid: 0.2-1 mL
- Formalin Fixed Paraffin-embedded Tissue (FFPE/PET): blocks are preferred and will be sent back to client upon completion of testing
- Scrolls/unstained slides: cross-sectional area >1cm^2 send 10 sections of 10µm thickness, if <1cm^2 send 20 sections if available
Please note: We do not need a separate specimen aliquot for each test ordered. Only a single specimen aliquot or block of optimal quantity is necessary for performing multiple tests. If multiple aliquots or blocks of optimal quantity are sent, up to 2 will be pooled.
Quantity
requested: See Optimal Quantity above
minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.
Processing
UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.
Freeze specimens at -20°C upon arrival
Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on dry ice.
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| UW-MT |
Microbiology, Molecular Diagnostics
206-520-4600 Clinical Lab, Room # NW177, |
|---|
Frequency
Fresh frozen tissues/fluids result in 2-3 business days after receipt of specimen. Formalin Fixed Paraffin-embedded tissues result in 3-4 business days after receipt of specimen.
Available STAT?
No