Enterobacterales DNA Detection

General Information

Lab Name

Enterobacterales DNA Detection

Lab Code

ENBDNA

Epic Name

(not orderable in Epic)

Description

Enterobacterales DNA Detection by PCR is only available as a reflexive test when results indicate the presence of bacteria in the Order Enterobacterales from Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA] or Bacterial detection by 16S NGS [NGS16S].

Results in 2-3 business days after reflex.

The family Enterobacteriaceae (within the order Enterobacterales) contains over 60 genera and more than 250 species, and organisms within this family include pathogenic bacteria capable of causing many different infectious processes, such as sepsis, pneumonia, urinary tract infections, intra-abdominal infections and foodborne illnesses. 16S gene sequencing, a gold standard for determining the species of a bacteria, however is not adequate due to strong conservation of sequences among members of this family across multiple genera. Instead, sequencing housekeeping genes such as dnaJ has been shown to distinguish species within this family.

We have designed Enterobacterales DNA Detection [ENBDNA] based on partial dnaJ gene sequencing which can be used to detect and identify members of the Order Enterobacterales. This test is currently offered reflexively when 16S sequence (from Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA] or Bacterial detection by 16S NGS [NGS16S]) suggests presence of order Enterobacterales DNA.

Synonyms

16S sequencing, bacteria 16S, bacteria identification, Bacteria PCR, bacteria sequencing, bacterial 16S, bacterial identification, Bacterial PCR, bacterial sequencing, broad range bacteria PCR, broad range bacterial PCR, dnaJ, dnaJ identification, dnaJ PCR, dnaJ sequencing, Enterobacteriaceae identification, Enterobacteriaceae sequencing, Family Enterobacteriaceae, molecular bacteria, molecular bacterial, Synonyms: 16S PCR, universal bacteria PCR, universal bacterial PCR

Components

Code Name
ENBRS Enterobacterales PCR: Detection, dnaJ
ENBID Enterobacterales PCR: dnaJ Identification
ENBSI Enterobacterales PCR: Specimen Description
ENBSPI Enterobacterales PCR: External Identifier
ENBSR Enterobacterales PCR: Special Requests
ENBSC Enterobacterales PCR: Specimen Comments
ENBNAE Enterobacterales PCR: Specimen DNA Extraction
ENBREV Enterobacterales PCR: Pathologist Review
ENBME Enterobacterales PCR: Method Note

Ordering & Collection

Specimen Type

This only available as a reflexive test, please refer to Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA]

Collection

Processing

This only available as a reflexive test, please refer to Bacterial PCR reflex NGS [BCTDNA], Bacterial DNA Detection by PCR (without reflex to NGS) [NRBDNA]

Performance

LIS Dept Code

Micro Molecular Diag (MMD)

Performing Location(s)

CL Molecular Microbiology
206-598-5735

Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW
Room 171A
Renton, WA 98057

Performing Lab Address

UW Medicine Central Laboratory
Molecular Microbiology
1601 Lind Ave SW
Room 485
Renton, WA 98057

We receive specimens 7 days a week, 365 days a year.

Please e-mail us with any questions or comments you have, so that we can provide you with the best possible service. Our e-mail is monitored in real time, and your inquiry will be answered as soon as possible.

e-mail: molmicdx@uw.edu

For urgent inquiries, billing inquiries, or requests for faxed reports, please contact our Client Services Department at (206) 598-5735 or (800) 713-5198.

Frequency

Results in 2-3 business days after reflex from Bacterial PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).

Available STAT?

No

Billing & Coding

CPT codes

87801

LOINC

76575-0

Interfaced Order Code

UOW4340