Enterobacterales DNA Detection

Enterobacterales DNA Detection by PCR is only available as a reflexive test when results indicate the presence of bacteria in the Order Enterobacterales from Bacterial DNA Detection by PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).

Results in 2-3 business days after reflex.

The family Enterobacteriaceae (within the order Enterobacterales) contains over 60 genera and more than 250 species, and organisms within this family include pathogenic bacteria capable of causing many different infectious processes, such as sepsis, pneumonia, urinary tract infections, intra-abdominal infections and foodborne illnesses. 16S gene sequencing, a gold standard for determining the species of a bacteria, however is not adequate due to strong conservation of sequences among members of this family across multiple genera. Instead, sequencing housekeeping genes such as dnaJ has been shown to distinguish species within this family.

We have designed an Enterobacterales PCR test (ENBDNA) based on partial dnaJ gene sequencing which can be used to detect and identify members of Enterobacterales order. This test is currently offered reflexively when 16S sequence (from BCTDNA or NGS16S) suggests presence of Enterobacteriaceae family DNA.

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website