Syphilis Serologic Confirmation (Sendout)
General Information
Lab Name
Syphilis Serologic Confirmation (Sendout)
Lab Code
RSYPHC
Epic Name
Syphilis Serologic Confirmation (Sendout)
Description
Ordering Note: For use by UW-MT Immunology Laboratory only. Reflex confirmation testing for borderline/reactive Serologic Syphilis Screen [SYPHSC] results.
Testing Algorithm: CMIA (detection of IgG and IgM antibodies by Abbott chemiluminescent Immunoassay) and RPR (Rapid Plasma Reagin, qualitative and quantitative) are performed on each specimen. If the results are inconclusive or discrepant between the two tests, a confirmatory TP-PA (Treponema pallidum Particle Agglutination) is performed.
Components
| Code | Name |
|---|---|
| RTCMIA | Syphilis Conf Treponemal CMIA, IgG/IgM |
| RRTPPA | Syphilis Conf TPPA |
| RPRQNT | Syphilis Conf RPR Quantitative Titer |
| RSYPPL | Syphilis Conf Performing Lab |
| SYPHIF | Final Syphilis Screening |
Interpretation
Method
Treponemal Specific Assays:
- CMIA (chemiluminescent Immunoassay): Detection of IgG and IgM antibodies by Abbott.
- TP-PA: (Treponema pallidum Particle Agglutination) Confirmation of IgG and IgM antibodies by Fujirebio.
Non-Treponemal Specific Assay:
- RPR: (Rapid Plasma Reagin) Qualitative and quantitative detection of non-specific antibodies by BD.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Orderable by reflex only.
Forms & Requisitions
Quantity
requested: 2 mL serum
minimum: 0.5 mL serum
Processing
Immunology: If RSYPHC reflexes from SYPHSC, aliquot extra serum into a separate vial (labeled with RSYPHC Sunquest CID). Freeze serum at -20°C.
Sendouts:
- Immunology will deliver serum aliquot with a completed WA PHL STI Requisition for sendout to the state lab.
- Package each patient's sample in an individual biohazard bag with a copy of the associated requisition (one patient per bag).
- Orders received with multiple patient samples/requisitions in the same biohazard bag may be rejected by WA PHL.
- Send samples on dry ice with the DLMP courier.
WAPHL Specimen Collection and Submission Instructions: Syphilis, Reference
Stability: Frozen: Indefinitely; Refrigerated: 5 days; Ambient: Unacceptable.
Rejection Criteria: Unacceptable specimen type, not refrigerated or frozen, insufficient specimen volume, leaky specimen.
Performance
LIS Dept Code
Serology Sendouts (SRSO)
Performing Location(s)
| Sendout |
Washington State Public Health Laboratory
206-418-5400 1610 NE 150th St. |
|---|
Other Locations/Notes
- WA PHL STI Lab: (206) 418-5622
Frequency
Monday - Friday, excluding holidays. TAT: CMIA and RPR: 3 business days. TP-PA: 5 business days.
Available STAT?
No