Treponema Pallidum DNA detection, Mucocutaneous
General Information
Lab Name
Treponema Pallidum DNA detection, Mucocutaneous
Lab Code
TPLSWB
Epic Name
Treponema pallidum DNA Detection, Mucocutaneous
Description
Detection of Treponema pallidum DNA.
This test detects Treponema pallidum, the etiologic agent of syphilis and endemic treponematoses (e.g., yaws and pinta) in mucocutaneous swabs. It can be ordered as a primary diagnostic when syphilis is suspected.
Please note that this assay does NOT distinguish subspecies of T. pallidum.
Synonyms
23S LAMP, 23S PCR, chancre, darkfield microscopy, FTA-ABS, meningovascular, neurosyphilis, RPR, spirochete, Syphilis, tabes dorsalis, TP-PA, TPPA, Treponema, Treponema pallidum, treponeme, VDRL
Components
| Code | Name |
|---|---|
| TPMC23 | T pallidum NAAT: Detection, 23S rDNA |
| TPMCID | T pallidum NAAT: Identification |
| TPMCSI | T pallidum NAAT: Specimen Description |
| TPMCSP | T pallidum NAAT: External Identifier |
| TPMCSR | T pallidum NAAT: Special Requests |
| TPMCSC | T pallidum NAAT: Specimen Comments |
| TPMCNA | T pallidum NAAT: Specimen DNA Extraction |
| TPMCRV | T pallidum NAAT: Pathologist Review |
| TPMCME | T pallidum NAAT: Method Note |
Interpretation
Method
DNA extraction, nucleic acid purification, Loop Mediated Isothermal Amplification
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Preferred specimen: Mucocutaneous swabs, including genital, oral, anal/rectal and lesions
Acceptable specimen: Mucocutaneous swabs, including genital, oral, anal/rectal and lesions
Unacceptable specimen:
- Citrated or heparinized solutions
- Tissues floating in formalin
- Swab/fluid collected in tube containing agar
Handling Instructions
Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.
Quantity
requested: 650uL
minimum: 500uL
Processing
UWMC/HMC: Store and send UTM swabs refrigerated or frozen. If specimen storage and transport will exceed 48 hours, freeze at -20 to -80°C. SPOT to route to first would be Virology Renton Annex
Performance
LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
| UW-MT |
Microbiology, Molecular Diagnostics
206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology Performing Lab Address Clinical Microbiology Lab, NW177 |
Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers:
For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190 |
|---|
Frequency
Mondays and Thursdays. Batched and currently performed 1 – 2 times per week.
Available STAT?
No