GC (N. gonorrhoeae) and CT (Chlamydia trachomatis) Amplified Nucleic Acid Detection

General Information

Lab Name

GC and Chlam Nucleic Acid Detection

Lab Code

GCCTAD

Epic Name

GC and Chlam Nucleic Acid Detection

Description

This testing is not intended for the evaluation of suspected sexual abuse or for other medico-legal indications. The performance of this test has NOT been evaluated in patients less than 14 years of age or in pregnant women.

NOTE: A specimen type MUST be provided - correct performance and interpretation of this test is limited to the specimen types and collection methods described in Collection and Handling.

Synonyms

Gonorrhea NAA, Neisseria-Amplification

Components

Code Name
GCCTSD GC and Chlam NA Spec Description
CTAD Chlam trachomatis Nucleic Acid
GCAD N.gonorrhoeae(GC) Nucleic Acid

Interpretation

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type

Vagina, Endocervix, Urethra, Throat, Anal, First void urine, Liquid Pap specimen

Collection

Note: Please indicate specimen type with all orders.

  • Endocervical or urethral specimen: Collect specimen with APTIMA UnisexCollection Kit (narrow-tipped blue swab into white tube with purple lettering). Remove cervical mucous with large white swab labeled APTIMA Cleaning Swab and discard before using blue swab to collect specimen. Tubes containing white swabs instead of blue will be rejected for testing. Specimens submitted in the APTIMA Multitest Collection Kit (orange tube with pink swab) will be accepted and performed with a suboptimal comment attached to the result.
  • Vagina: Collect specimen using the APTIMA Multitest Collection Kit (orange tube with pink swab). Specimens submitted in the Aptima Unisex Collection Kit (narrow-tipped blue swab into white tube with purple lettering) will be accepted and performed with a suboptimal comment attached to the result.
  • Throat: Collect specimen using the APTIMA Multitest Collection Kit (orange tube with pink swab). Specimens submitted in the Aptima Unisex Collection Kit (narrow-tipped blue swab into white tube with purple lettering) will be accepted and performed with a suboptimal comment attached to the result.
  • Anal/Rectal: Collect specimen using the APTIMA Multitest Collection Kit (orange tube with pink swab). Avoid collecting stool on the swab. Specimens submitted in the Aptima Unisex Collection Kit (narrow-tipped blue swab into white tube with purple lettering) will be accepted and performed with a suboptimal comment attached to the result.
  • Urine: Collect 10 to 30 mL of first stream of first morning void (Dirty Catch for maximal sensitivity) or first stream at least 1 hour after last void in urine cup. Submit urine cup or transfer 2 mL of urine with the disposable pipette to the yellow tube included in the APTIMA Urine Collection Kit.
  • Liquid Pap Specimen: Transfer PreservCyt Solution liquid Pap specimen into the Aptima Specimen Transfer tube (green label)

Unacceptable specimens: Catheterized urine, frozen urine, or unpreserved urine received >24 hours after collection, gel-based culture swab, Eswab, or any Aptima tube containing the white cleaning swab.

Handling Instructions

Swab or Urine in APTIMA tube: Transport under ambient conditions within 30 days.

  1. Aptima tubes remain stable at both ambient temperature and under refrigeration.
  2. Unpreserved urine samples are acceptable for up to 24 hours, whether stored at ambient temperature or refrigerated.
  3. Once transferred to the Aptima collection kits, specimens are stable for up to 2 months when stored between 2–30 °C

Unpreserved urine: Transport under ambient conditions within 24 hours; specimens received after 24 hours will be rejected.

Quantity

requested: Swab in proper Aptima tube, 2 mL urine transferred to yellow Aptima tube, 20 mL unpreserved urine in cup, 1 mL of PreservCyt Solution liquid Pap specimen in Aptima Specimen Transfer tube
minimum: Swab in APTIMA tube, 2 mL urine in APTIMA urine tube, or 20 mL urine in cup, 1 mL of PreservCyt Solution liquid Pap specimen in APTIMA Specimen Transfer tube

Processing

Alert! Unpreserved urine must be transferred to an APTIMA urine tube within 24 hours of collection.

Login:

Order CHLAD if only CT (R/O Chlamydia trachomatis) is requested.

Order GCCAD if only GC (R/O Neisseria gonorrhoeae) is requested.

Order GCCTAD when R/O both GC and CT are requested.

Performance

LIS Dept Code

Serology (SR)

Performing Location(s)

HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency

Set up: 6 days a week, Monday - Saturday

Available STAT?

No

Billing & Coding

CPT codes

87494

LOINC

64017-7

Interfaced Order Code

UOW102