Respiratory Virus PCR Panel (no SARS-CoV-2)
General Information
Lab Name
Respiratory Virus PCR Panel (no SARS-CoV-2)
Lab Code
REVSQF
Epic Name
Respiratory Virus PCR Panel (no SARS-CoV-2)
Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and identification of multiple respiratory viral pathogens.
The following viruses are targeted (gene):
Adenovirus, AdV (Hexon)
human Metapneumovirus, hMPV (nucleocapsid)
Rhinovirus, RV (5” UTR)
Influenza A, FluA (matrix)
Influenza B, FluB (matrix)
Respiratory Syncytial Virus, RSV (matrix)
Human Parainfuenza virus type 1, hPIV1 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 2, hPIV2 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 4, hPIV3 (hemagglutinin neuraminidase)
Human Parainfuenza virus type 4, hPIV4 (hemagglutinin neuraminidase)
Coronavirus (CoV) subtypes OC43 (ORF1ab)
Coronavirus (CoV) subtypes HKU1 (ORF1ab)
Coronavirus (CoV) subtypes NL63 (Spike)
Coronavirus (CoV) subtypes 229R (ORF2ab)
Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.
The performance characteristics of the test were determined by the University of Washington Department of Laboratory Medicine and Pathology. The modified version has not been cleared or approved by the US Food and Drug Administration.
For ordering individual battery components:
FUSAMR, Adeno/Metapneumo/Rhino Virus PCR Panel [FUSAMR]
FLURSV, Influenza A, B & RSV PCR Panel [FLURSV]
FUSPIV, Parainfluenza PCR Panel [FUSPIV]
Components
Interpretation
Method
Real-time Polymerase Chain Reaction.
Reference Range
See individual components
Guidelines
Ordering & Collection
Specimen Type
Collection
Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.
Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.
Minimum sample requirement: 0.75 mL
Handling Instructions
Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity
requested: 1.0 mL
minimum: 0.75 mL
Processing
Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.
Performance
LIS Dept Code
Performing Location(s)
| CL |
Virology, Renton
206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356 |
206-685-6656 opt 4 |
|---|
Frequency
Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?
No