Lymphocyte subsets, T, B, NK cell counts

General Information

Lab Name

Lymphocyte subsets, T, B, NK cell counts

Lab Code

TCSNK

Epic Name

T Cell Subsets w/NK Cells

Description

Notice:

The UW Hematopathology Laboratory is currently being impacted by a global supply shortage of a critical component of our Lymphocyte Subset Enumeration assays. Until reagent is made available by the manufacturer, lymphocyte subset enumeration assays listed below will be sent to a referral laboratory for testing as early as Friday, September 23. To ensure sample viability for testing, please schedule collections so that samples are received in our lab on weekdays and no later than noon on Fridays. Samples received later than noon on Friday or over the weekend will have an increased risk for cancellation due to limited stability.

The following tests are affected:

· T cell subsets CD4/CD8 only, counts [TCS48]

· T cell subsets CD3/CD4/CD8, counts [TCSA]

· Lymphocyte subsets, T and B cell counts [TCSP]

· Lymphocyte subsets, T, B, NK cell counts [TCSNK]

· CD19+ B Cell Count [BCS19]

This assay provides absolute cell counts and percentages for CD4, CD8, CD3 T cell subsets, CD19 B cell subsets, and NK cells.

All lymphocyte subset panels offered:
T cell subsets CD4/CD8 only, counts [TCS48] - CD4, CD8
T cell subsets CD3/CD4/CD8, counts [TCSA] - CD4, CD8, CD3
Lymphocyte subsets, T and B cell counts [TCSP] - CD4, CD8, CD3, CD19
Lymphocyte subsets, T, B, NK cell counts [TCSNK] - CD4, CD8, CD3, CD19, CD56
CD19+ B Cell Count [BCS19] - CD19 only

References

https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360

Synonyms

CD4, CD8, NK, T Cell Subsets plus Natural Killer Cells

Components

Code Name
CD4P % CD4
CD4A Abs CD4 Lymphocyte Cnt
CD8P % CD8
CD8A Abs CD8 Lymphocyte Cnt
CD19P % CD19
CD19A Abs CD19 Lymphocyte Count
3NNKPP % CD3_, CD16+56+
3NNKPA Abs CD3_CD16+/56+ Lymph Count
CD4CD8 CD4/CD8 Ratio
CD3P % CD3
CD3A Abs CD3 Lymphocyte Cnt
NFDA4 Test Comment

Interpretation

Method

This is a single platform lymphocyte subset assay and has been approved by the U.S. Food and Drug Administration.

Reference Range

See individual components

Ref. Range Notes

%CD4 [33-61]
Abs CD4 [0.730-2.250] Thou/ul
%CD8 [14-35]
Abs CD8 [0.250-1.240] Thou/ul
CD4/CD8 [1.00-3.78]
%CD3 [57-87]
Abs CD3 [1.040-3.400] Thou/ul
%CD19 [9-23]
Abs CD19 [0.160-0.390] Thou/ul
%NK [2-27]
Abs NK [0.027-0.693]

Interferences and Limitations

The stability limit for this FDA approved assay is 24 hours

Guidelines

Ordering & Collection

Specimen Type

Blood

Collection

3 or 5 mL Lavender (EDTA) tube
Unacceptable: GEL SEPARATOR tubes

Handling Instructions

Specimens must be tested within 24 hours of collection for this FDA assay. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Specimens can be transported at room temperature.

Quantity

requested: 3-4 ml
minimum: 1 ml

Processing

Stability: 24 hours @Room Temperature

SPS: Sample can be shared with CBC. Do not add on test if sample will be received in the Hematopathology lab outside the 24-hour stability limit. If sample is approaching 24 hour stability limit, contact hematopathology lab (206-606-7060) to notify them that a specimen is coming and send immediately.

Outside Laboratories: Specimens must be tested within 24 hours of collection. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Contact Lab Med Call Center 206-685-6066 to discuss transportation options. Specimens can be sent directly to the hematopathology lab as well (include contact info).

Performance

LIS Dept Code

Hematopathology (HP)

Performing Location(s)

Other Hematopathology
206-606-7060

UW Hematopathology Laboratory, G7-800
Seattle Cancer Care Alliance
825 Eastlake Avenue E.
Seattle, WA 98109

Frequency

Monday-Sunday. For same day testing, sample must arrive in Hematopathology lab by 5pm Monday - Saturday and 2:30pm on Sunday. Testing is offered on the following holidays: Veteran's Day, MLK, Day after Thanksgiving. NOT offered on: President's day, Memorial day, 4th of July, Labor day, Thanksgiving, Christmas, or New Year’s Day.

Available STAT?

No

Billing & Coding

CPT codes

LOINC

18768-2