Lymphocyte subsets, T, B, NK cell counts
General Information
Lab Name
Lymphocyte subsets, T, B, NK cell counts
Lab Code
TCSNK
Epic Name
T Cell Subsets w/NK Cells
Description
This assay provides absolute cell counts and percentages for CD4, CD8, CD3 T cell subsets, CD19 B cell subsets, and NK cells.
All lymphocyte subset panels offered:
T cell subsets CD4/CD8 only, counts [TCS48] - CD4, CD8
T cell subsets CD3/CD4/CD8, counts [TCSA] - CD4, CD8, CD3
Lymphocyte subsets, T and B cell counts [TCSP] - CD4, CD8, CD3, CD19
Lymphocyte subsets, T, B, NK cell counts [TCSNK] - CD4, CD8, CD3, CD19, CD56
CD19+ B Cell Count [BCS19] - CD19 only
References
https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.21360
Synonyms
CD4, CD8, NK, T Cell Subsets plus Natural Killer Cells
Components
| Code | Name |
|---|---|
| CD4P | % CD4 |
| CD4A | Abs CD4 Lymphocyte Cnt |
| CD8P | % CD8 |
| CD8A | Abs CD8 Lymphocyte Cnt |
| CD19P | % CD19 |
| CD19A | Abs CD19 Lymphocyte Count |
| 3NNKPP | % CD3_, CD16+56+ |
| 3NNKPA | Abs CD3_CD16+/56+ Lymph Count |
| CD4CD8 | CD4/CD8 Ratio |
| CD3P | % CD3 |
| CD3A | Abs CD3 Lymphocyte Cnt |
| NFDA4 | Test Comment |
Interpretation
Method
This is a single platform lymphocyte subset assay and has been approved by the U.S. Food and Drug Administration.
Reference Range
See individual components
Ref. Range Notes
| %CD4 | [33-61] |
| Abs CD4 | [0.730-2.250] Thou/ul |
| %CD8 | [14-35] |
| Abs CD8 | [0.250-1.240] Thou/ul |
| CD4/CD8 | [1.00-3.78] |
| %CD3 | [57-87] |
| Abs CD3 | [1.040-3.400] Thou/ul |
| %CD19 | [9-23] |
| Abs CD19 | [0.160-0.390] Thou/ul |
| %NK | [2-27] |
| Abs NK | [0.027-0.693] |
Interferences and Limitations
The stability limit for this FDA approved assay is 24 hours
Guidelines
Ordering & Collection
Specimen Type
Collection
3 or 5 mL Lavender (EDTA) tube
Unacceptable: GEL SEPARATOR tubes
Handling Instructions
Specimens must be tested within 24 hours of collection for this FDA assay. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Specimens must be transported at room temperature. Refrigerated or frozen specimens will be canceled and a re-draw will be requested.
Quantity
requested: 3-4 ml
minimum: 1 ml
Processing
Stability: 24 hours @Room Temperature
SPS: Sample can be shared with CBC. Do not add on test if sample will be received in the Hematopathology lab outside the 24-hour stability limit. If sample is approaching 24 hour stability limit, contact hematopathology lab (206-606-7060) to notify them that a specimen is coming and send immediately.
Outside Laboratories: Specimens must be tested within 24 hours of collection. For samples received > 24 hours after collection, test will be canceled and a re-draw will be requested. Contact Lab Med Call Center 206-685-6066 to discuss transportation options. Specimens can be sent directly to the hematopathology lab as well (include contact info).
Performance
LIS Dept Code
Hematopathology (HP)
Performing Location(s)
| Other |
Hematopathology
206-606-7060 UW Hematopathology Laboratory, G7-800 |
Hematopathology hours: |
|---|
Frequency
Monday-Sunday.
Available STAT?
No