Research Sequencing Service (RSS)

Welcome to the Department of Laboratory Medicine and Pathology (DLMP) Research Sequencing Service (RSS). The RSS is designed as a partnership between research-oriented faculty with the DLMP Virology lab to take advantage of spare sequencing capacity in order to facilitate the use of cutting-edge sequencing technologies in the DLMP. We are located in the E-wing of the University of Washington’s Health Sciences Building and are staffed by a team with a decade of hands-on experience in developing and using the Illumina Next Generation Sequencing platforms.

Director: Scott R Kennedy, PhD; 
Computational Support: Brendan F. Kohrn, MS;

The RSS supports several sequencing pipelines, from tissue or single cells to analysis. The specific supported analysis pipelines can be found on the Supported Analysis Pipelines page. The current available equipment includes a 10x Genomics Chromium X controller for single-cell library generation and cell encapsulation, an Agilent TapeStation 4200 for quality control, and access to an Illumina MiSeq (capable of 1 million to 25 million read pairs), NextSeq2000 (capable of 400 million to 1.1 billion read pairs), or NovaSeq6000 (capable of 800 million to 10 billion read pairs). The final data output can be analyzed by a full featured range of applications on a DLMP-hosted processing server and delivered to the investigator. More information can be found on the Supported Analysis Pipelines page.

The RSS provides all the equipment needed to perform all the pipeline steps. The end-user only needs to provide consumables such as plastics, reagents, and the kits and cartridges from 10x, Agilent, and Illumina.

For further information on how to take advantage of this resource and estimated reagent costs, please refer to the Forms and Resources page and fill out the required forms.